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hcr hybridization buffer  (Molecular Instruments)

 
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    Molecular Instruments hcr hybridization buffer
    Hcr Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcr+hybridization+buffer/amplification+buffer/bio_rxiv__64898__2026__04__28__719500-607-6-9
    Average 86 stars, based on 1 article reviews
    hcr hybridization buffer - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Hybridization:

    Article Title: Multimodal alignments of in vivo imaging and spatial biology datasets at cellular resolution
    Article Snippet: .. We then replaced the medium with HCR hybridization buffer (Molecular Instruments), incubated the slices in 37 °C for 10 min, added the probes at their optimized concentrations in the hybridization buffer, and incubated the slices overnight. .. The next day, we washed the hybridized slices with HCR washing buffer (Molecular Instruments) at 37 °C for 10 min, and repeated this washing step three times.

    Article Title: The GABA A receptor RDL modulates the auditory sensitivity of malaria mosquitoes
    Article Snippet: .. Sections were then prehybridized with prewarmed HCR hybridization buffer (Molecular Instruments, USA) for 40 min at 37°C. ..

    Article Title: Multi-gene biomarkers reveal spatial organization and subpopulation-specific damage response in intrahepatic biliary epithelial cells
    Article Snippet: .. Prewarmed (37°C) HCR Hybridization Buffer (Molecular Instruments, v3.0) was added to each slide and incubated for 15min at 37°C. ..

    Article Title: Force coordination distinguishes epithelial and mesenchymal modes of collective chemotaxis
    Article Snippet: .. Sections were equilibrated in HCR hybridization buffer (Molecular Instruments) for 5 min at 37°C and incubated overnight at 37°C with the twist probe set diluted in pre-warmed hybridization buffer. ..

    Article Title: Retinoic acid coordinates the orderly construction of the mammalian body in the anterior-to-posterior sequence
    Article Snippet: .. Prior to hybridization, samples were equilibrated in HCR hybridization buffer (Molecular Instruments). ..

    Article Title: Engulfment by brain macrophages in a short-lived vertebrate
    Article Snippet: After washing off OCT, slides were then permeabilized in 1% Triton (e.g., Sigma-Aldrich T8787-50ML) in 100% methanol (e.g., Sigma-Aldrich 34860-100ML-R) for at least 1 hour at −20°C, then washed twice in RNase-free 2x saline sodium citrate (SSC; diluted from 20x SSC (ThermoFisher, AM9763) in RNase-free water) with 0.1% Triton (250 μL per slide) for 15 minutes per wash at room temperature. .. HCR hybridization buffer (Molecular Instruments, “HCR Probe Hybridization Buffer,” for tissue sections; 250 μL per slide; preheated to 37°C) was applied to the slides, which were incubated at 37°C for 30 minutes with a coverslip on top. ..

    Article Title: Tissue-resident macrophage and dendritic cells drive type I IFN immunity to enteroviruses in the liver.
    Article Snippet: .. Sections were treated with 10 μg/mL proteinase K for 10 minutes at 37°C, then incubated with HCR hybridization buffer (Molecular Instruments) for 10 minutes at 37°C. ..

    Article Title: Tissue-resident macrophage and dendritic cells drive type I IFN immunity to enteroviruses in the liver
    Article Snippet: .. Sections were treated with 10 μg/mL proteinase K for 10 minutes at 37°C, then incubated with HCR hybridization buffer (Molecular Instruments) for 10 minutes at 37°C. ..

    Incubation:

    Article Title: Multimodal alignments of in vivo imaging and spatial biology datasets at cellular resolution
    Article Snippet: .. We then replaced the medium with HCR hybridization buffer (Molecular Instruments), incubated the slices in 37 °C for 10 min, added the probes at their optimized concentrations in the hybridization buffer, and incubated the slices overnight. .. The next day, we washed the hybridized slices with HCR washing buffer (Molecular Instruments) at 37 °C for 10 min, and repeated this washing step three times.

    Article Title: Multi-gene biomarkers reveal spatial organization and subpopulation-specific damage response in intrahepatic biliary epithelial cells
    Article Snippet: .. Prewarmed (37°C) HCR Hybridization Buffer (Molecular Instruments, v3.0) was added to each slide and incubated for 15min at 37°C. ..

    Article Title: Force coordination distinguishes epithelial and mesenchymal modes of collective chemotaxis
    Article Snippet: .. Sections were equilibrated in HCR hybridization buffer (Molecular Instruments) for 5 min at 37°C and incubated overnight at 37°C with the twist probe set diluted in pre-warmed hybridization buffer. ..

    Article Title: Engulfment by brain macrophages in a short-lived vertebrate
    Article Snippet: After washing off OCT, slides were then permeabilized in 1% Triton (e.g., Sigma-Aldrich T8787-50ML) in 100% methanol (e.g., Sigma-Aldrich 34860-100ML-R) for at least 1 hour at −20°C, then washed twice in RNase-free 2x saline sodium citrate (SSC; diluted from 20x SSC (ThermoFisher, AM9763) in RNase-free water) with 0.1% Triton (250 μL per slide) for 15 minutes per wash at room temperature. .. HCR hybridization buffer (Molecular Instruments, “HCR Probe Hybridization Buffer,” for tissue sections; 250 μL per slide; preheated to 37°C) was applied to the slides, which were incubated at 37°C for 30 minutes with a coverslip on top. ..

    Article Title: Tissue-resident macrophage and dendritic cells drive type I IFN immunity to enteroviruses in the liver.
    Article Snippet: .. Sections were treated with 10 μg/mL proteinase K for 10 minutes at 37°C, then incubated with HCR hybridization buffer (Molecular Instruments) for 10 minutes at 37°C. ..

    Article Title: Tissue-resident macrophage and dendritic cells drive type I IFN immunity to enteroviruses in the liver
    Article Snippet: .. Sections were treated with 10 μg/mL proteinase K for 10 minutes at 37°C, then incubated with HCR hybridization buffer (Molecular Instruments) for 10 minutes at 37°C. ..



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    hcr hybridization buffer  (Molecular Instruments)
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    Molecular Instruments hcr hybridization buffer
    Hcr Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hcr probe hybridization buffer  (Molecular Instruments)
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    Molecular Instruments hcr probe hybridization buffer
    Example of multiplex whole-mount RNA fluorescent <t>HCR</t> in situ <t>hybridization</t> combined with immunohistochemistry in the mosquito brain (A) Whole mosquito brain showing RNA fluorescent HCR in situ hybridization against putative GAD1 ( AGAP005866 ) and SerT ( AGAP010621 ) and immunohistochemistry against PDF and DAPI. (B–B″″) Maximum projection (∼2 μm total) of the optic lobe showing non-overlapping cell bodies of GAD1 + and SerT + cells and PDF neurites. Individual panels show fluorescence channels imaged using the following excitation wavelengths: 488 nm (PDF), 546 nm ( GAD1 ), 647 nm ( SerT ), and 358 nm (DAPI). See also and .
    Hcr Probe Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcr+hybridization+buffer/buffer+hybridization+probe/pmc12509983-13-0-5
    Average 86 stars, based on 1 article reviews
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    hcr hifi probe hybridization buffer  (Molecular Instruments)
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    Molecular Instruments hcr hifi probe hybridization buffer
    (A,B-A””,B””) <t>Hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> was performed on coronal sections of E10.5 mandibular processes to assess gene expression patterns for Dlx5 (green) (A-B), Dlx6 (cyan) (A’-B’), Hand2 (magenta) (A”-B”), Sox10 (yellow) (A”’-B”’) in controls and double mutants. (A””-B””) Merged image of A-A”’ and B-B”’ with the counterstain DAPI in blue. (C) RT-qPCR for the indicated mRNA transcripts isolated from the mandibular processes of control and Prdm3;Prdm16 double mutants at E10.5 (n = 4 for both control and mutants). (D) RT-qPCR for Sox10 at E10.5 (n = 4 for both control and mutants) and E11.5 (n = 3 for control, n = 4 for mutants). (E-F, E’-F’) HCR was performed on coronal sections of E11.5 mandibular processes to assess gene expression patterns for Sox10 . (E’-F’) Merged image of E and F with the counterstain DAPI in blue. Double white arrowhead denotes the initial presumptive cranial ganglia and peripheral nerve tracks entering the mandibular prominence in control animals. White arrow indicates areas of Sox10 expression that are expanded in the mesenchyme in the Prdm3;Prdm16 double mutants. Abbreviations: MdP, mandibular process. *P≤0.05; (unpaired, two-tailed Student’s t test).
    Hcr Hifi Probe Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Example of multiplex whole-mount RNA fluorescent HCR in situ hybridization combined with immunohistochemistry in the mosquito brain (A) Whole mosquito brain showing RNA fluorescent HCR in situ hybridization against putative GAD1 ( AGAP005866 ) and SerT ( AGAP010621 ) and immunohistochemistry against PDF and DAPI. (B–B″″) Maximum projection (∼2 μm total) of the optic lobe showing non-overlapping cell bodies of GAD1 + and SerT + cells and PDF neurites. Individual panels show fluorescence channels imaged using the following excitation wavelengths: 488 nm (PDF), 546 nm ( GAD1 ), 647 nm ( SerT ), and 358 nm (DAPI). See also and .

    Journal: STAR Protocols

    Article Title: Protocol for multiplex whole-mount RNA fluorescence in situ hybridization combined with immunohistochemistry in the mosquito brain

    doi: 10.1016/j.xpro.2025.104109

    Figure Lengend Snippet: Example of multiplex whole-mount RNA fluorescent HCR in situ hybridization combined with immunohistochemistry in the mosquito brain (A) Whole mosquito brain showing RNA fluorescent HCR in situ hybridization against putative GAD1 ( AGAP005866 ) and SerT ( AGAP010621 ) and immunohistochemistry against PDF and DAPI. (B–B″″) Maximum projection (∼2 μm total) of the optic lobe showing non-overlapping cell bodies of GAD1 + and SerT + cells and PDF neurites. Individual panels show fluorescence channels imaged using the following excitation wavelengths: 488 nm (PDF), 546 nm ( GAD1 ), 647 nm ( SerT ), and 358 nm (DAPI). See also and .

    Article Snippet: HCR probe hybridization buffer , Molecular Instruments , N/A.

    Techniques: Multiplex Assay, In Situ Hybridization, Immunohistochemistry, Fluorescence

    (A,B-A””,B””) Hybridization chain reaction (HCR) was performed on coronal sections of E10.5 mandibular processes to assess gene expression patterns for Dlx5 (green) (A-B), Dlx6 (cyan) (A’-B’), Hand2 (magenta) (A”-B”), Sox10 (yellow) (A”’-B”’) in controls and double mutants. (A””-B””) Merged image of A-A”’ and B-B”’ with the counterstain DAPI in blue. (C) RT-qPCR for the indicated mRNA transcripts isolated from the mandibular processes of control and Prdm3;Prdm16 double mutants at E10.5 (n = 4 for both control and mutants). (D) RT-qPCR for Sox10 at E10.5 (n = 4 for both control and mutants) and E11.5 (n = 3 for control, n = 4 for mutants). (E-F, E’-F’) HCR was performed on coronal sections of E11.5 mandibular processes to assess gene expression patterns for Sox10 . (E’-F’) Merged image of E and F with the counterstain DAPI in blue. Double white arrowhead denotes the initial presumptive cranial ganglia and peripheral nerve tracks entering the mandibular prominence in control animals. White arrow indicates areas of Sox10 expression that are expanded in the mesenchyme in the Prdm3;Prdm16 double mutants. Abbreviations: MdP, mandibular process. *P≤0.05; (unpaired, two-tailed Student’s t test).

    Journal: Developmental biology

    Article Title: PRDM paralogs are required for Meckel’s cartilage formation during mandibular bone development

    doi: 10.1016/j.ydbio.2025.12.002

    Figure Lengend Snippet: (A,B-A””,B””) Hybridization chain reaction (HCR) was performed on coronal sections of E10.5 mandibular processes to assess gene expression patterns for Dlx5 (green) (A-B), Dlx6 (cyan) (A’-B’), Hand2 (magenta) (A”-B”), Sox10 (yellow) (A”’-B”’) in controls and double mutants. (A””-B””) Merged image of A-A”’ and B-B”’ with the counterstain DAPI in blue. (C) RT-qPCR for the indicated mRNA transcripts isolated from the mandibular processes of control and Prdm3;Prdm16 double mutants at E10.5 (n = 4 for both control and mutants). (D) RT-qPCR for Sox10 at E10.5 (n = 4 for both control and mutants) and E11.5 (n = 3 for control, n = 4 for mutants). (E-F, E’-F’) HCR was performed on coronal sections of E11.5 mandibular processes to assess gene expression patterns for Sox10 . (E’-F’) Merged image of E and F with the counterstain DAPI in blue. Double white arrowhead denotes the initial presumptive cranial ganglia and peripheral nerve tracks entering the mandibular prominence in control animals. White arrow indicates areas of Sox10 expression that are expanded in the mesenchyme in the Prdm3;Prdm16 double mutants. Abbreviations: MdP, mandibular process. *P≤0.05; (unpaired, two-tailed Student’s t test).

    Article Snippet: Sections were pre-hybridized in HCR HiFi Probe Hybridization Buffer (Molecular Instruments) for 10 minutes at 37°C in a humidified chamber.

    Techniques: Expressing, Hybridization, Gene Expression, Quantitative RT-PCR, Isolation, Control, Two Tailed Test